Introduction
Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged nuclei acids, proteins and lipids. RNA and DNA are probably the most biologically significant targets of oxidative attack. Recently, oxidative damage to RNA molecules has been described in several neurological diseases including Alzheimer’s disease, Parkinson’s disease, Down syndrome, dementia with Lewy bodies, prion disease, subacute sclerosing panencephalitis, and xeroderma pigmentosum. Among numerous types of RNA oxidative damage, the formation of 8-hydroxyguanosine (8-OHG) is a ubiquitous marker of oxidative stress.
Cell Biolabs’ Oxidative RNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-OHG in urine, serum, cerebrospinal fluid or other cell or tissue RNA samples. The quantity of 8-OHG in unknown sample is determined by comparing its absorbance with that of a known 8-OHG standard curve. The kit has an 8-OHG detection sensitivity range of 300 pg/mL to 40 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.
Assay Principle
The Oxidative RNA Damage ELISA kit is a competitive ELISA for the quantitative measurement of 8-OHG. The unknown 8-OHG samples or 8-OHG standards are first added to an 8-OHG/BSA conjugate preabsorbed EIA plate. After a brief incubation, an anti-8-OHG monoclonal antibody is added, followed by an HRP conjugated secondary antibody. The 8-OHG content in unknown samples is determined by comparison with predetermined 8-OHG standard curve.
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